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Toyopharl chromatographic separation packing is based on methacrylic acid polymer, which is used for large-scale separation and preparation. The polymer skeleton is hard and has better pressure flow characteristics than other products on the market. Therefore, the linear flow rate can be increased, the preparation time can be accelerated and the cycle time can be reduced. Tsk-gel high-pressure chromatographic separation and purification packing includes ion exchange and hydrophobic separation modes; toyopharl medium and low-pressure chromatographic separation and purification packing includes size exclusion, ion exchange, hydrophobic, affinity and other separation modes. The analysis results of tskgel pre column with the same chemical group can be used to predict the possibility of large-scale amplification of toyoperarl packing. This allows us to seamlessly enlarge the method developed by tskgel column to the large-scale separation and purification of toyoperarl packing.